Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: Expressing, Staining

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: In Situ

Journal: Neuroscience Bulletin

Article Title: Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells

doi: 10.1007/s12264-019-00353-0

Figure Lengend Snippet: Protein levels of mGluR1/5 and NMDAR subunits in retinal extracts from Rac1-cKO, Chat-cre+/–, and control mice. A Representative immunoblots showing the mGluR1 and mGluR5 protein levels. B Bar charts summarizing the average densitometry of immunoreactive bands of mGluR1 and mGluR5 expression. C Representative immunoblots showing the GluN1, GluN2A, and GluN2B protein levels. D Bar charts summarizing the average densitometry of immunoreactive bands of GluN1, GluN2A, and GluN2B expression. All the data are normalized to control. n = 6–7. *P < 0.05, ***P < 0.001 vs control.

Article Snippet: After blocking in 5% non-fat milk at room temperature for 2 h, the membranes were incubated overnight at 4°C with the following primary antibodies: polyclonal mouse anti-GluN1 (1:1000; BD Pharmingen, Franklin Lakes, NJ), polyclonal rabbit anti-GluN2A (1:200; Alomone Labs, Jerusalem, Israel), polyclonal rabbit anti-GluN2B (1:200; Alomone Labs), polyclonal rabbit anti-GluA1 (1:200; Alomone Labs), monoclonal rabbit anti-mGluR1 (1:1000; Cell Signaling Technology, Danvers, MA), monoclonal rabbit anti-mGluR5 (1:500; Abcam), polyclonal rabbit anti-glycine receptor alpha1+alpha2 (1:1000; Abcam), and polyclonal rabbit anti-GABA A receptor alpha1 (GABRA1) (1:1000; Abcam).

Techniques: Western Blot, Expressing

Journal: AIDS Research and Treatment

Article Title: Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma

doi: 10.1155/2010/164586

Figure Lengend Snippet: AIDS-NHL cell lines express CXCR5, as shown by flow cytometry. The AIDS-BL cell line, 2F7 (a), and the AIDS-DLBCL cell line, R (b), were stained for CXCR5 expression using an indirect staining protocol, as noted in . First, cells were stained with a rat IgG 2b anti-CXCR5 antibody (dotted lines). As a control, some cells were stained with a rat IgG 2b isotype control antibody (solid lines). Cells were then stained with a PE-conjugated goat anti-rat IgG secondary antibody, and examined by flow cytometry. During the flow cytometry acquisition stage, at least 5,000 cells/events were acquired per tube/condition. During analysis, dead cells were excluded using forward- and side-scatter.

Article Snippet: For CXCR5, a rat antihuman CXCR5 antibody (clone RF8B2, R&D Systems) or IgG 2b isotype control (clone KLH/G2b-1-2, Southern Biotechnology Associates, Birmingham, AL) was used (2 μ g/mL, 2 hours).

Techniques: Flow Cytometry, Staining, Expressing, Control

Journal: Cell reports

Article Title: Genetic and Functional Dissection of the Role of Individual 5-HT 2 Receptors as Entry Receptors for JC Polyomavirus

doi: 10.1016/j.celrep.2019.04.067

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-5-HT 2B R was obtained from Novus Biologicals cat# NLS1111 and recognizes the second extracellular loop.

Techniques: Generated, Virus, Recombinant, Mutagenesis, Luciferase, Control, Plasmid Preparation, Software, CRISPR

Journal: Molecular Therapy

Article Title: Enterovirus A71 Oncolysis of Malignant Gliomas

doi: 10.1016/j.ymthe.2020.04.005

Figure Lengend Snippet: Roles of Virus Receptor and Apoptosis in EV-A71-Mediated Oncolysis

Article Snippet: IHC For IHC, the sections were incubated with primary rabbit anti-EV-A71 2B antibody (GTX132343; GeneTex), followed by reaction with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (ab7090; Abcam).

Techniques: Virus

Journal: Molecular Therapy

Article Title: Enterovirus A71 Oncolysis of Malignant Gliomas

doi: 10.1016/j.ymthe.2020.04.005

Figure Lengend Snippet: PMAIP1 Induction Is Involved in EV-A71-Mediated Oncolysis

Article Snippet: IHC For IHC, the sections were incubated with primary rabbit anti-EV-A71 2B antibody (GTX132343; GeneTex), followed by reaction with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (ab7090; Abcam).

Techniques:

Journal: Molecular Therapy

Article Title: Enterovirus A71 Oncolysis of Malignant Gliomas

doi: 10.1016/j.ymthe.2020.04.005

Figure Lengend Snippet: EV-A71 Oncolytic Activity against Glioma Xenografts

Article Snippet: IHC For IHC, the sections were incubated with primary rabbit anti-EV-A71 2B antibody (GTX132343; GeneTex), followed by reaction with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (ab7090; Abcam).

Techniques: Activity Assay

Journal: Molecular Therapy

Article Title: Enterovirus A71 Oncolysis of Malignant Gliomas

doi: 10.1016/j.ymthe.2020.04.005

Figure Lengend Snippet: Construction and Characterization of Recombinant miR124-Regulated EV-A71

Article Snippet: IHC For IHC, the sections were incubated with primary rabbit anti-EV-A71 2B antibody (GTX132343; GeneTex), followed by reaction with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (ab7090; Abcam).

Techniques: Recombinant

Journal: Molecular Therapy

Article Title: Enterovirus A71 Oncolysis of Malignant Gliomas

doi: 10.1016/j.ymthe.2020.04.005

Figure Lengend Snippet: Antitumor Activity of miR124-Sensitive EV-A71 against Gliomas

Article Snippet: IHC For IHC, the sections were incubated with primary rabbit anti-EV-A71 2B antibody (GTX132343; GeneTex), followed by reaction with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (ab7090; Abcam).

Techniques: Activity Assay